Figure 1

Reduction of cell survival in patient-derived lymphoma cells exposed to HDM2 SMIs. Box plots show percent survival compared to control for isolated primary lymphoma cells following exposure to increasing concentrations of MI-219 and Nutlin-3 at indicated time points. Survival is expressed as a percentage of live cells detected by Trypan blue exclusion relative to the total number of cells from each of the 11 patients ranging from 2 to 6 replicates per patient. The horizontal lines within the boxes represent the median while the upper and lower lines of the box endorse the 25th and 75th interquartile range (IQR). The upper- and lower-most lines extend to cover points within 1.5 times the IQR and circles outside of the lines indicate outliers. A mixed effects analysis of variance was used where the drug, concentration and time were defined as fixed effects; patient and replication were defined as random effects. Holm’s procedure was used to adjust for multiple comparisons. Significant overall differences between MI-219 and Nutlin-3 treatments were observed (p < 0.001).