Figure 3

Targeting cord formation or pericyte association in the ADSC/ECFC co-culture model. (a) CD31 stained basal or VEGF stimulated cords at 3 days treated with 2 μM Sunitinib, 20 μg/mL bevacizumab (Bev), or 20 μg/mL ramucirumab (Ram). (b) Graphs of a concentration response of sunitinib, bevacizumab (Bev), and ramucirumab (Ram) on total tube area of basal and VEGF stimulated cord formation after 3 days. (c) VEGF stimulated cords were established for 4 days then treated with control or a PDGFR-β inhibitor, IMC-2C5 (2C5) for 3 days. Images show the effects of control and IMC-2C5 on VEGF stimulated endothelial cords stained with CD31 (green) and SMA-positive pericytes (red). (d) Graphs of total tube area (left) and SMA index (right) of VEGF stimulated cords with or without PDGFR-β inhibition. n = 3 per group. * = p < 0.001 vs. Control. † = p < 0.01 vs. VEGF.