Differential responses to genotoxic agents between induced pluripotent stem cells and tumor cell lines

Lu, Yinghua; Xu, Dazhong; Zhou, Jing; Ma, Yupo; Jiang, Yongping; Zeng, Wenxian; Dai, Wei

doi:10.1186/1756-8722-6-71

Journal of Hematology & Oncology

Table 1 Time-dependent responses of human iPS, Tera-1, and BEAS-2B cells after treatment with Cr(VI), H ₂ O _2, or Dox

From: Differential responses to genotoxic agents between induced pluripotent stem cells and tumor cell lines

Cell type	Treatment	Total p53	p-ATM	γH2AX	p-p53^S15	p-p53^S20	p-p53^S392
iPS cells	Cr(VI)	Up early down late	Biphasic induction	Strongly up late	Unchanged	Undetectable	Undetectable
	10 μM	Up early down late	Biphasic induction	Strongly up late	Unchanged	Undetectable	Undetectable
	H₂O₂	Slightly up early and down late	Biphasic induction	Strongly up late	Strongly up early and down late	Undetectable	Slightly up early and down late
	100 μM	Slightly up early and down late	Biphasic induction	Strongly up late	Strongly up early and down late	Undetectable	Slightly up early and down late
	Dox	Slightly up over time	Up over time	Up late	Up early and down late	Undetectable	Strongly up early and down late
	250 nM	Slightly up over time	Up over time	Up late	Up early and down late	Undetectable	Strongly up early and down late
Tera-1	Cr(VI)	Slightly up over time	Up late	Strongly up late	Strongly up overtime	Undetectable	Strongly up over time
	10 μM	Slightly up over time	Up late	Strongly up late	Strongly up overtime	Undetectable	Strongly up over time
	H₂O₂	Slightly up over time	Up early and down late	Undetectable	Unchanged	Up over time and down late	Up early and stays up
	100 μM	Slightly up over time	Up early and down late	Undetectable	Unchanged	Up over time and down late	Up early and stays up
	Dox	Significantly up over time	Significantly up early and down late	Strongly up at 8 h and down late	Strongly up late	Slightly up late	Strongly up over time
	250 nM	Significantly up over time	Significantly up early and down late	Strongly up at 8 h and down late	Strongly up late	Slightly up late	Strongly up over time
BEASE-2B	Cr(VI)	Unchanged	Slightly up over time	Strongly up late	Up over time	Up over time	Unchanged
	10 μM	Unchanged	Slightly up over time	Strongly up late	Up over time	Up over time	Unchanged
	H₂O₂	Unchanged	Largely unchanged	Undetectable	Largely unchanged	Largely unchanged	Largely unchanged
	100 μM	Unchanged	Largely unchanged	Undetectable	Largely unchanged	Largely unchanged	Largely unchanged
	Dox	Unchanged	Up early and down late	Strongly up late	Strongly up early and down late	Strongly up early and down late	Unchanged up to 24 h and down at 48 h
	250 nM	Unchanged	Up early and down late	Strongly up late	Strongly up early and down late	Strongly up early and down late	Unchanged up to 24 h and down at 48 h

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ISSN: 1756-8722

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