Fig. 1

AGK is upregulated in RCC and is associated with poor prognosis in 120 RCC patients. a Representative images of AGK protein expression in 12 pairs of RCC tissues (T) and adjacent normal tissues (N). b Statistical analysis of the relative AGK protein levels in 12 pairs of RCC tumour samples and adjacent normal tissues. c Relative AGK RNA expression in 12 pairs of RCC tumour samples and adjacent normal tissues. d AGK protein and e mRNA expression were detected in a normal cell line (HK-2) and seven renal cell lines (Caki-1, Caki-2, 786O, A498, SK-RC-39, 769P and ACHN). GAPDH was used as a loading control. The error bars represent the standard deviation of the mean (SD) calculated from three experiments performed in parallel. P values were calculated using an independent Student’s t test. *P <  0.05 versus control. f Representative IHC images showing AGK expression in RCC tissue and adjacent normal tissue. g Kaplan-Meier analysis of overall survival (OS) and h distant metastasis-free survival (DMFS) according to AGK expression in 120 RCC patients