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Figure 4 | Journal of Hematology & Oncology

Figure 4

From: Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells

Figure 4

CFU and LTC- IC expansion fold under different culture conditions. A: CFU expansion fold under different culture conditions: After 14 days culture, cells were harvested from different culture conditions and put to Methocult H4435 medium for another 2 weeks. CFU-GM, BFU-E/CFU-E and CFU-GEMM were counted according to their morphologic characters. Comparison of absolute CFU (total CFU together with three different types of CFU) expansion fold after 14 days culture with or without alemtuzumab treatment is shown. The results of three separate experiments performed in duplicate are expressed in term of the CFU expansion fold on day 14 compared to day 0 initiating cells. B: LTC-IC expansion fold under different culture conditions: LTC-IC was performed on cells before and after 14 days of culture and, in the absence or presence of alemtuzumab. Results are from 3 separate experiments from performed in triplicate on separate fresh cord blood samples.

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