Figure 4

CK19 and HER2 RNA expression from serial immunomagnetic selection of peripheral blood mononuclear cells (PBMC) from 4 normal subjects. Subpopulations of PBMC from 4 normal subjects (C, D, E, F) were isolated by serial immunomagnetic selection with CD14 (monocytes), CD3/CD19 (lymphocytes), and CD16 (natural killer cells/granulocytes). An additional PBMC sample from each subject underwent immunomagnetic selection with BerEP4 (epithelial cells) as a negative control. MDA-MB-361 RNA was used for standard curves when detecting CK19 and HER2. RNA from normal leukocytes was used for standard curve for B2M detection. End cells: cells remaining after the serial selection. Panel A. CK19 signal detected using quantitative real-time RT-PCR and expressed as cell equivalents of MDA-MB-361 from epithelial cell and mononuclear cell subfractions. Panel B. HER2 signal detected using quantitative real-time RT-PCR and expressed as cell equivalents of MDA-MB-361 from epithelial cell and mononuclear cell subfractions.