Table 1 Oligonucleotides used in the study.
Name of oligonucleotide | Sequences of the oligonucleotide |
|---|---|
1. Gel shifting Oct-1 oligos. | |
Oct-1 binding probe (sense strand) | 5'-CTGATACGATTTGCATACTGACGT-3' |
Oct-1 binding probe (anti-sense strand) | 3'-GACTATGCTAAACGTATGACTGCA-5' |
2. Decoy oligonucleotides | |
Oct-1 Decoy oligo (sense strand) | 5'-TGTCGA ATGCAAAT CACTAGAA-3' |
Oct-1 Decoy oligo (anti-sense strand) | 3'-ACAGCT TACGTTTA GTGATCTT-5' |
3. Control oligonucleotides. | |
Scrambled oligo (sense strand) | 5'-AGTCGTCACGTAAGTCGAGCAC-3' |
Scrambled oligo (anti-sense strand) | 3'-TCAGCAGTGCATTCAGCTCGTG-5' |
4. Real time PCR primers. | |
Forward primer | 5'-TGGTGACCGTTTTGGCAATC-3' |
Reverse primer | 5'-GAAAGCTCTGCATCATGGGC-3' |