Figure 6

Haptoglobin genotyping. Hp genotyping based on combination of the results of two separate DNA amplification reactions involving primers A and B in the first reaction (lanes 2, 4, 6) and primers C and D in the second reaction (lanes 3, 5, 7). The reactions in lanes 2 and 3 contained DNA from the individual with genotype Hp 1-1; the reactions in lanes 4 and 5 contained DNA from the individual with genotype Hp 2-1; the reactions in lanes 6 and 7 contained DNA from the individual with genotype Hp 2-2. Lane 1, DNA size marker 100 – 1500 bp (Genscrip); lane 2, allele Hp 1; lane 3, no amplification product was obtained with the Hp 2-specific primer pair C/D because this sample was homozygous for allele Hp 1; lane 4, allele Hp 1; lane 5, allele Hp 2; lane 6, allele Hp 2; lane 7, allele Hp 2; lane 8, DNA size marker 1 kb plus (Gibco).