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Figure 1 | Journal of Hematology & Oncology

Figure 1

From: Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR

Figure 1

IB RT-PCR sensitivity test on dilutions of MDA-MB-453 in normal blood. Cells were added to 10 mls of normal blood. Lane 1, M = pUC 19/Hpa II marker. Lanes 2–4, no added cells. Lanes 5–7, 10 added cells. Lanes 8–10, 100 added cells, Lanes 11–13, 1000 added cells. Lane 14, C = cDNA positive control. Lane 15, R = RT negative control. Lanes 16–18, N = PCR negative controls. Product size is shown in base pairs (bp). A genomic DNA band is seen above the RT-PCR band for ELF3. The legend shows cells/10 ml of blood.

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Journal of Hematology & Oncology

ISSN: 1756-8722

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