Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR

Table 1 RT-PCR primers and PCR conditions.

Primer name	GenBank Accession Number	Sequence 5' – 3'	PCR annealing temperature	Size
ELF3 s	AF016295	CTCGGAGCTCCCACTCCTCAGA
ELF3 as		GCTCTTCTTGCCCTCGAGACAGT	68°C	188 bp
EPHB4 s	AB209644	CCCCAGGGAAGAAGGAGAGCTG
EPHB4 as		GCCCACGAGCTGGATGACTGTG	68°C	250 bp
EGFR s	AB209442	TGTGAGGTGGTCCTTGGGAATTTGG
EGFR as		TGCTGACTATGTCCCGCCACTGGA	66°C	339 bp
TACSTD1 s	BC014785	GGACCTGACAGTAAATGGGGAAC
TACSTD1 as		CTCTTCTTTCTGGAAATAACCAGCAC	68°C	186 bp
MGB1 s	AY217100	CGGATGAAACTCTGAGCAATGTTGAG
MGB1 as		CTGCAGTTCTGTGAGCCAAAGGTC	68°C	110 bp

Sequences are shown for sense (s) and antisense (as) primers. The annealing temperatures used for the PCR reactions and the PCR product sizes are also shown.

ISSN: 1756-8722