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Figure 1 | Journal of Hematology & Oncology

Figure 1

From: Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications

Figure 1

IFN-γ- or CD137-based enrichment of antigen-specific immune effector cells. (A) CD4 and CD8 T cell distribution in IFN-γ- or CD137-positive cell population after antigen stimulation. The ratios of CD4 to CD8 T cells of five donors were presented. (B) and (C) CD137 expression in CD4 and CD8 T cells before and after Ab affinity column purification. PBMC were mixed with EBV LMP2 pentadecapeptides and 3–24 hr later, IFN-γ-secreting cells or CD137-positive cells were isolated by using MACS magnetic bead affinity columns as described in Materials and Methods. The cells were stained with PE-conjugated anti-IFN-γ or anti-CD137 Ab and CD3, CD4 and CD8 specific Ab and analyzed with flow cytometry.

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