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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications

Figure 2

Phenotype analysis of IFN-γ- or CD137-selected antigen-specific immune cells. PBMC were stimulated with EBV LMP2A pentadecapeptides and IFN-γ or CD137 positive cells were isolated for analysis. (A) & (B) Memory and effector phenotype analysis based on seven-color flow cytometry with surface staining for CD27, CD28, CD45RA, CCR7, CD4, CD8, CD137 and intracellular staining for IFN-γ immediately after cell isolation without further culture. The percentage of different populations (IFN-γ or CD137 plus CD4 or CD8 gated) of T cells is illustrated for a CD4-dominant donor (A) and a CD8-dominant donor (B); Tcm, central memory T cells (CD27+/-, CD28+, CCR7+, CD45RA-); Tem, effector memory T cells (CD27+/-, CD28+/-, CCR7-, CD45RA-); Teff, terminal effector T cells (CD27-, CD28-, CCR7+/-, CD45RA+).

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