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Figure 3 | Journal of Hematology & Oncology

Figure 3

From: Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications

Figure 3

Phenotype analyses of IFN-γ- or CD137-selected immune effector cells after ex vivo expansion. (A) Phenotype analysis after ex vivo expansion. After the rapid selection, the antigen-specific immune cells were cultured for fifteen days with irradiated autologous PBMC as feeder cells. The distribution of CD3, CD4, CD8 and CD56 cell populations was determined and representative FACS graphs are shown. (B) and (C) Memory and effector phenotype analysis based on seven-color flow cytometry with surface staining for CD27, CD28, CD45RA, CCR7, CD4, CD8, CD137 and intracellular staining for IFN-γ. Representative results of a CD4-dominant donor (B) and a CD8-dominant donor (C) are shown.

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