Figure 4
Effector function analyses of IFN-γ- or CD137-selected immune effector cells after ex vivo expansion. (A) and (B) Flow cytometry analysis of IFN-γ and CD137 expression after restimulation of the culture expanded IFN-γ or CD137 effector cells. The cells were restimulated with different cells as indicated and subjected to antibody staining and flow cytometry analysis; control, WT-1 peptide-pulsed DC; specific, LMP2A peptide-pulsed DC; BLCL, autologous EBV-transformed B cells. (C) Analysis of antigen-specific cytolytic activity. The ex vivo expanded IFN-γ- or CD137-enriched cells were incubated with autologous EBV transformed B cells (BLCL), mature DCs loaded with either LMP2A pentadecapeptides (DC-LMP2A) or WT1 pentadecapeptides (DC-WT1, as control) at 1:1 ratio in a cytotoxicity assay based on CFSE labeling as described in Materials and Methods.