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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications

Figure 6

Ex vivo expansion and phenotype analyses of DC-activated immune effector cells. EBV LMP2A-specific effector T cells were generated by stimulation of non-adherent PBMC with DC pulsed with LMP2A pentadecapeptides. (A) Growth kinetics of DC-activated immune effector cells. The viable cells were counted with trypan blue staining at different time points after coculture and the growth curves of 5 samples are shown. (B) and (C) CD3, CD56, CD4 and CD8 phenotype analysis. The DC-activated cells from day 0, 12 and 19 were stained with antibodies against CD4, CD8, CD3 and CD56 and analyzed with flow cytometry. The percentages of different lymphocyte subsets were analyzed and shown in bar graphs. Donor 1*, PBMC collected at a different time point from donor 1.

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