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Figure 8 | Journal of Hematology & Oncology

Figure 8

From: Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications

Figure 8

Functional analyses of DC-activated and ex vivo expanded immune effector cells. EBV LMP2A-specific effector cells were generated by stimulation of non-adherent PBMC with DC pulsed with LMP2A pentadecapeptides. (A) Analysis of antigen-specific effector cytokines and CD107a expression. The DC-activated immune cells from day 19 coculture were stimulated with autologous DC pulsed with LMP2A peptides, WT1 peptides (non-specific control) or no stimulation (IE cell alone) for 6 hours. The cells were stained with Ab against CD4, CD8, CD107a and IFN-γ. Flow cytometry analyses of IL-2, IFN-γ and CD107a-positive cells in CD4-gated or CD8-gated populations were illustrated as representative of five experiments. (B) Comparison of cytolytic function of IFN-γ-selected (same donor as in Fig. 4C) versus DC-activated LMP2-specific immune effector cells. The LMP2A-specific effector cells were mixed with target cells including autologous BLCL, DC-LMP2A, or control DC-WT1 at 1:1 ratio and analyzed for cytolytic activity based on the CFSE-labeling method as described in Materials and Methods.

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