Basic Mechanisms of Arsenic Trioxide (ATO)-Induced Apoptosis in Human Leukemia (HL-60) Cells

Table 2 Summary data of caspase-3 assay obtained from the flow cytometry analysis.

ATO Concentrations	Caspase-3 Negative Cells or Viable Cells	Caspase-3 Positive Cells or Apoptotic Cells
	(Mean ± SD)%	(Mean ± SD)%
0 μg/mL	98.7 ± 0.6	1.1 ± 0.3
2 μg/mL	82.5 ± 8.9*	17.5 ± 8.9*
4 μg/mL	63.0 ± 2.4*	27.0 ± 2.4*
6 μg/mL	37.5 ± 8.8*	62.5 ± 8.8*
8 μg/mL	36.9 ± 9.7*	63.1 ± 9.7*

HL-60 promyelocytic leukemia cells were cultured in the absence or presence of ATO for 24 h as indicated in the Materials and Methods. Values are shown as means ± SDs of 3 replicates per experiment. *Significantly different at p < 0.05 to the control group.

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