Table 1 Primers for RT-PCR analysis
From: Lobaplatin arrests cell cycle progression in human hepatocellular carcinoma cells
Gene | Forward primer | Reverse primer |
|---|---|---|
cyclin B | GCACTTTCCTCCTCCTCAA | CTTCGATGTGGCCATCTTG |
cyclin D1 | CTGTGCTGCGAAGTGGAAACCAT | TTCATGGCCAGCGGGAAGACCTC |
CDK1 | GATTCTATCCCTCCTGGTC | TAGGCTTCCTGGTTTCC |
CDK4 | CTGAGAATGGCTACCTCTCGATATG | AGAGTGTAACAACCACGGGTGTAAG |
CDK6 | CCGAGTAGTGCATCGCGATCTAA | CTTTGCCTAGTTCATCGATATC |
CDC25C | GAACAGGCCAAGGCTGAAGC | GCCCCTGGTTAGAATCTTCC |
p53 | GAGGCGCTGCCCCCACCATGA | AGCTCTCGGAACATCTCGAAGC |
p16 | AGCCTTCGGCTGACTGGCTGG | CTGCCCATCATCATGACCTGG |
p21 | TTAGGGCTTCCTCCTGGAGGAGAT | ATGTCAGAACCGGCTGGGGATGTC |
p27 | CCTCTTCGGCCCGGTGGAC | TTTGGGGAACCGTCTGAAAC |
GAPDH | GGGAAGGTGAAGGTCGGAGTC | AGCAGAGGGGGCAGA |