Figure 2

Features of the expression of TNFSF10 , BCL2L1 , SPP1 , and CREBBP genes in T-ALL and healthy groups. A, B: Accurate standard curve graphs of BCL2L1 and the β2M control gene are shown using diluted Molt-4 cDNA as the template. The amplification efficiency of BCL2L1-related genes was more than 95%, and consistent with the high amplification efficiency of the β2M reference gene. C: Melting curves of the BCL2L1 and β2M genes from nine patients. #: Specific peak of the β2M reference gene begins at 81°C. ##: Specific peak of the BCL2L1 gene begins at 84°C. D: PCR products of the β2M gene by 2.5% agarose gel electrophoresis analysis. The size of the PCR products of the β2M gene used for the BCL11B reference is 332 bp (line 1, 2) and that used for the four genes of interest is 145 bp (line 4-11). Line 3: DNA ladder. E: PCR products analyzed by 2.5% agarose gel electrophoresis. Line 1-2: BCL11B (193bp), line 3: DNA ladder, line 4-5: BCL2L1 (202 bp), line 6-7: CREBBP (206 bp), line 8-9: SPP1 (241 bp), line 10-11: TNFSF10 (190 bp). F: Relative expression levels of the four genes of interest in T-ALL and healthy groups.