Figure 2
From: Dysregulation of miR-15a and miR-214 in human pancreatic cancer
MiR-15a and miR-214 have different roles in pancreatic cancer cells. (A) qRT-PCR was used to investigate miRNA transfection efficiency. Both miR-15a and miR-214 were significantly increased compared to their mimics-NC (control) in BxCP-3 cells. (B) The viability of BxCP-3 cells after transfection was measured by CCK-8 assay. (C) Cell viability was measured using the CCK-8 assay in BxCP-3 cells treated with 10 μM GEM at 24, 48 and 72 hrs. (D) The expression pattern of miR-214 was detected by qRT-PCR in BxCP-3 cells treated with GEM. (E) The CCK-8 assay was used to measure the inhibition effect of miR-214 on apoptosis of BxCP-3 cells induced by GEM. BxCP-3 cells were transfected with H2O (MOCK), mimics-NC (NC), and miR-214 mimics (miR-214). Significant differences (* p < 0.05; ** p < 0.01) compared with the control were calculated using Dunnett's test or the paired Student's t-test.