Dysregulation of miR-15a and miR-214 in human pancreatic cancer

Table 3 Target validation for miR-214

miR-214 target		Synthesized 3'-UTR containing the predicted MRE	MRE validated by luciferase activity	Specially suppressed by miR-214 mimics
PIM1	F	TCGAGTACTTGAACTTGCCTCTTTTACCTGCTGCTTCTCCAAAAATCTGCCTGGGTTGC	YES	NT
	R	GGCCGCAACCCAGGCAGATTTTTGGAGAAGCAGCAGGTAAAAGAGGCAAGTTCAAGTAC
RASSF5	F	TCGAGCTCCCTTTAGAAACTCTCTCCCTGCTGTATATTAAAGGGAGCAGGTGGAGAGC	YES	NT
	R	GGCCGCTCTCCACCTGCTCCCTTTAATATACAGCAGGGAGAGAGTTTCTAAAGGGAGC
BAX	F	TCGAGTGATCAATCCCCGATTCATCTACCCTGCTGACCTCCCAGTGACCCCTGACCTGC	YES	NT
	R	GGCCGCAGGTCAGGGGTCACTGGGAGGTCAGCAGGGTAGATGAATCGGGGATTGATCAC
BIK	F	TCGAGACCACTGCCCTGGAGGTGGCGGCCTGCTGCTGTTATCTTTTTAACTGTTTTCGC	YES	NT
	R	GGCCGCGAAAACAGTTAAAAAGATAACAGCAGCAGGCCGCCACCTCCAGGGCAGTGGTC
NEO1	F	TCGAGTGTGTCGAGGCAGCTTCCCTTTGCCTGCTGATATTCTGCAGGACTGGGCACCGC	YES	NT
	R	GGCCGCGGTGCCCAGTCCTGCAGAATATCAGCAGGCAAAGGGAAGCTGCCTCGACACAC
ING4	F	TCGAGGTAAATAAAAGCTATACATGTTGGCCTGCTGTGTTTATTGTAGAGACACTGTGC	YES	YES
	R	GGCCGCACAGTGTCTCTACAATAAACACAGCAGGCCAACATGTATAGCTTTTATTTACC
ACVR1B	F	TCGAGTCATTGGGGGGACCGTCTTTACCCCTGCTGACCTCCCACCTATCCGCCCTGCGC	YES	NT
	R	GGCCGCGCAGGGCGGATAGGTGGGAGGTCAGCAGGGGTAAAGACGGTCCCCCCAATGAC

The 59-bp segments of the 3'-UTR of the target genes are listed in the table. F (forward sequence) and R (reverse sequence) were annealed together and inserted into the psi-CHECK-control vector. NT, negative.

ISSN: 1756-8722