Figure 1

PHI reversed hypermethylation of p15 and induced transcription activation. A: PHI induced p15 hypomethylation with the decreasing of the methylated p15 (p15-M) and increase of the unmethylated form of p15 (p15-U). MS-PCR was performed using primers specific for p15-M and p15-U DNA forms of p15 as described in the methods. 5-Aza (2 μM) and TSA (1 μM) were used as controls for DNA hypomethylation. B: P15 mRNA levels were enhanced by PHI treatment. The expression levels of p15 mRNA were measured by RT-PCR. C: Up-regulation of p15 protein expression by PHI. P15 protein levels were detected by Western blotting, and β-actin was used as a loading control.