Table 1 PCR premiers for P15, DNMT1, DNMT3A, and DNMT3B mRNA
| sequence (5'-3') | extent (bp) | anneal temperature (°C) | |
|---|---|---|---|
| actin1 | F:gtggggcgccccaggcacca | 517 | Variable |
| R:ctccttaatgtcacgcacgatttc | |||
| actin2 | F:ctacaatgagctgcgtgtggc | 271 | Variable |
| R:caggtccagacgcaggatggc | |||
| p15 | F:tgggggcggcagcgatgag | 451 | 56 |
| R:aggtgggtgggggtgggaaat | |||
| DNMT1 | F:accatcacatctcattttgc | 238 | 56 |
| R:ggtttgacttcggagtctct | |||
| DNMT3A | F:cacacagaagcatatccaggagtg | 551 | 55 |
| R:agtggactgggaaaccaaataccc | |||
| DNMT3B | F:aatgtgaatccagccaggaaaggc | 190 | 55 |
| R:actggattacactccaggaaccgt |