Reactivating aberrantly hypermethylated p15 gene in leukemic T cells by a phenylhexyl isothiocyanate mediated inter-active mechanism on DNA and chromatin

Table 1 PCR premiers for P15, DNMT1, DNMT3A, and DNMT3B mRNA

	sequence (5'-3')	extent (bp)	anneal temperature (°C)
actin1	F:gtggggcgccccaggcacca	517	Variable
	R:ctccttaatgtcacgcacgatttc
actin2	F:ctacaatgagctgcgtgtggc	271	Variable
	R:caggtccagacgcaggatggc
p15	F:tgggggcggcagcgatgag	451	56
	R:aggtgggtgggggtgggaaat
DNMT1	F:accatcacatctcattttgc	238	56
	R:ggtttgacttcggagtctct
DNMT3A	F:cacacagaagcatatccaggagtg	551	55
	R:agtggactgggaaaccaaataccc
DNMT3B	F:aatgtgaatccagccaggaaaggc	190	55
	R:actggattacactccaggaaccgt

ISSN: 1756-8722