Figure 9
Effect of ZEA on cytosolic (A) and mitochondrial (B) Ca2+ level in HL-60 cells. HL-60 cells were incubated with Fluo3 (cytosolic) or Rhod2 (mitochondrial) Ca2+- specific dye for 15 min after treatment with and without ZEA for 1 h, then were subjected to flow cytometry as described in Materials and Methods. Black trace, control cells; red trace, ZEA-treated cells. Histogram of FACS analysis represents one of three independent experiments.