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Table 2 Identified Plasma Membrane Protein Spots in 24 h ZEA-treated HL-60 Cells by LC/MS/MS.

From: Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells

Spot no.

Protein Name(s)

Description

MW/pI

Peptide match

% Coverage

Sequence

*Expression in treated cells (folds)

1

TERA_HUMAN

Transitional endoplasmic reticulum ATPase

89.3/5.18

-

-

-

-1.99

2

GFAP_HUMAN

Glial fibrillary acidic protein

49.8/5.25

1

2.55

(K)LALDIEIATYR(K)

-1.55

 

K2C8_HUMAN

Keratin, type II cytoskeletal 8

53.7/5.34

1

2.28

  
 

HS90A_HUMAN

Heat shock protein HSP 90-alpha

83.2/4.97

7

10.00

K.IDIIPNPQER.T

K.EDQTEYLEER.R

K.HFSVEGQLEFR.A

R.RAPFDLFENK.K

R.GVVDSEDLPLNISR.E

K.FYEAFSK.N

K.EGLELPEDEEEK.K

 

3

GRP78_HUMAN

78 kDa glucose-regulated protein precursor (GRP 78)

72.3/5.10

-

-

-

1.93

4

PLSL_HUMAN

L-plastin, Lymphocyte cytosolic protein 1

70.2/5.02

11

22.01

(K)AACLPLPGYR(V)

(K)IGLFADIELSR(N)

(R)NEALIALLR(E)

(K)LSPEELLLR(W)

(K)AYYHLLEQVAPK(G)

(R)QFVTATDVVR(G)

(K)LNLAFIANLFNR(Y)

(R)VNHLYSDLSDALVIFQLYEK(I)

(K)FSLVGIGGQDLNEGNR(T)

(R)YTLNILEEIGGGQK(V)

(K)VNDDIIVNWVNETLR(E)

-3.1

5

CALR_HUMAN

Calreticulin precursor

60.6/4.37

-

-

-

2.39

6

PDIA1_HUMAN

Protein disulfide isomerase precursor

51.1/4.78

-

-

-

2.86

7

EF2_HUMAN

Elongation factor 2

95.1/6.78

-

-

-

-2.87

8

gi|28317

unnamed protein product

59.5/5.17

3

6.00

R.ALEESNYELEGK.I

R.QSVEADINGLR.R

R.NVQALEIELQSQLALK.Q

2.26

9

DHSA_HUMAN

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

72.6/7.04

4

8.43

(R)AAFGLSEAGFNTACVTK(L)

(R)GVIALCIEDGSIHR(I)

(K)NTVVATGGYGR(T)

(R)LGANSLLDLVVFGR(A)

-1.29

 

TCPG_HUMAN

T-complex protein 1 subunit gamma

60.5/6.06

1

2.02

(K)TAVETAVLLLR(I)

 

10

SERA_HUMAN

D-3-phosphoglycerate dehydrogenase

56.6/6.28

1

2.44

(K)GTIQVITQGTSLK(N)

-1.34

 

TCPZ_HUMAN

T-complex protein 1 subunit zeta

58.0/6.22

1

2.26

(K)GIDPFSLDALSK(E)

 
 

gi|4502643

chaperonin containing TCP1, subunit 6A isoform a

58.0/6.23

7

15.00

R.AQAALAVNISAAR.G

K.QADLYISEGLHPR.I

R.IITEGFEAAK.E

K.ALQFLEEVK.V

K.SETDTSLIR.G

K.GIDPFSLDALSK.E

K.VLAQNSGFDLQETLVK.I

 
 

gi|1002923

coronin-like protein

51.0/6.12

7

15.00

R.HVFGQPAK.A

R.EPVVTLEGHTK.R

R.AVFVSEGK.I

K.ILTTGFSR.M

R.DAGPLLISLK.D

R.AAPEASGTPSSDAVSR.L

K.LQATVQELQK.R

 

11

119623333

apoptosis inducing factor like isoform CRA d Homo sapiens

63.7/10.23

1

1.21

(R)LLSATSR(T)

2.18

 

RN112_HUMAN

RING finger protein 112

68.3/8.45

1

1.11

(R)LSGRYPK(V)

 
 

gi|4557014

catalase [Homo sapiens]

59.7/6.90

12

28.00

K.ADVLTTGAGNPVGDK.L

K.LNVITVGPR.G

K.GAGAFGYFEVTHDITK.Y

R.FR.DPILFPSFIHSQK.R

STVAGESGSADTVR.D

K.NLSVEDAAR.L

R.LSQEDPDYGIR.D

R.DLFNAIATGK.Y

R.LFAYPDTHR.H

K.DAQIFIQK.K

K.NFTEVHPDYGSHIQALLDK.Y

K.NAIHTFVQSGSHLAAR.E

 
 

gi|28317

unnamed protein product

59.5/5.17

7

14.00

R.ALEESNYELEGK.I

K.YENEVALR.Q

R.QSVEADINGLR.R

K.ADLEMQIESLTEELAYLK.K

R.NVQALEIELQSQLALK.Q

K.QSLEASLAETEGR.Y

R.LENEIQTYR.S

 

12

SAM50_HUMAN

Sorting and assembly machinery component 50 homolog

51.9/6.46

5

14.50

(K)VNQELAGYTGGDVSFIK(E)

(K)EDFELQLNK(Q)

(R)THFFLNAGNLCNLNYGEGPK(A)

(R)WSYGAGIVLR(L)

(R)ICDGVQFGAGIR(F)

-1.98

 

gi|7022134

unnamed protein product

51.9/6.62

9

20.00

K.DVVVQHVHFDGLGR.T

K.VTFQFSYGTK.E

R.NFSVNLYK.V

K.VTGQFPWSSLR.E

K.WEGVWR.E

K.VNQELAGYTGGDVSFIK.E

K.EDFELQLNK.Q

R.FYLGGPTSVR.G

R.WSYGAGIVLR.L

 
 

gi|4929571

CGI-51 protein

52.1/6.85

10

26.00

K.DVVVQHVHFDGLGR.T

K.VTFQFSYGTK.E

R.NFSVNLYK.V

K.VTGQFPWSSLR.E

K.WEGVWR.E

K.VNQELAGYTGGDVSFIK.E

K.EDFELQLNK.Q

K.QLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSIR.G

R.FYLGGPTSIR.G

R.WSYGAGIVLR.L

 
 

ANX11_HUMAN

Annexin A11

54.3/7.53

5

11.00

R.GTITDAPGFDPLR.D

K.TPVLFDIYEIK.E

R.LLISLSQGNR.D

R.SETDLLDIR.S

K.SLYHDISGDTSGDYR.K

 

13,14

ENOA_HUMAN

Alpha-enolase

47.0/7.54

-

-

-

-1.57,

-1.88

15

119571303

spectrin domain with coiled coils 1 isoform CRA d Homo sapiens

28.9/4.97

1

4.20

(R)LQIVSLASWAR(A)

5.14

 

ATPG_HUMAN

ATP synthase subunit gamma, mitochondrial

33.0/9.56

1

4.03

(R)IYGLGSLALYEK(A)

 
 

TPM3_HUMAN

Tropomyosin alpha-3 chain

32.8/4.49

1

2.82

(K)HIAEEADR(K)

 
 

ES8L1_HUMAN

Epidermal growth factor receptor kinase substrate 8-like protein 1

80.3/5.66

1

0.69

(K)SGPSR(K)

 
 

gi|16877071

ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1

32.9/9.23

3

11.00

R.IYGLGSLALYEK.A

K.HLLIGVSSDR.G

K.ELIEIISGAAALD.-

 

16

LDHB_HUMAN

L-lactate dehydrogenase B chain

36.6/5.64

2

8.08

(K)SLADELALVDVLEDK(L)

(R)VIGSGCNLDSAR(F)

-1.62

 

AFF4_HUMAN

AF4/FMR2 family member 4

12.7/9.68

1

0.77

(K)NSSSTSKQK(K)

 

17

COMT_HUMAN

Catechol O-methyltransferase

30.0/5.12

2

14.02

(K)VTLVVGASQDIIPQLK(K)

(K)GTVLLADNVICPGAPDFLAHVR(G)

1.07

 

PODXL_HUMAN

Podocalyxin like protein 1 precursor

55.6/5.23

1

2.46

(R)LASVPGSQTVVVK(E)

 
 

121944562

immunoglobulin A heavy chain variable region Homo sapiens

11.9/5.64

1

5.50

(K)VDGIEK(Y)

 
 

TRM13_HUMAN

tRNA guanosine-2'-O-methyltransferase TRM13 homolog

54.2/8.01

1

2.49

(R)KTSLETSNSTTK(R)

 

18

ANXA1_HUMAN

Annexin A1

38.7/6.63

5

22.00

K.GGPGSAVSPYPTFNPSSDVAALHK.A

K.GVDEATIIDILTK.R

K.ALTGHLEEVVLALLK.T

K.TPAQFDADELR.A

K.GTDVNVFNTILTTR.S

3.25

 

CN102_HUMAN

UPF0614 protein C14orf102

13.2/7.60

1

0.52

(R)LISLAK(C)

 

19

SOCS4_HUMAN

Suppressor of cytokine signaling 4

50.6/6.64

1

1.36

(R)SDLAFR(W)

-3.12

 

K2C1_HUMAN

Keratin, type II cytoskeletal 1(CK-1)

65.8/8.16

4

5.00

R.QFSSR.S

K.AEAESLYQSK.Y

K.YEELQITAGR.H

K.LALDLEIATYR.T

 
 

K2C7_HUMAN

Keratin, type II cytoskeletal 7 (CK-7)

51.2/5.50

1

2.00

K.LALDIEIATYR.K

 

20

gi|189054178

unnamed protein product [Homo sapiens]

66.0/7.62

4

6.00

R.SLDLDSIIAEVK.A

K.YEELQITAGR.H

K.LNDLEDALQQAK.E

R.TLLEGEESR.M

-2.84

21

AF047368_1

nebulette Homo sapiens

11.6/7.98

1

0.99

(K)ENQGNISSVK(Y)

2.99

 

ERp29_HUMAN

Endoplasmic reticulum protein ERp29

29.0/6.77

7

22.00

K.GALPLDTVTFYK.V

K.GALPLDTVTFYK.V

K.FVLVK.F

R.DGDFENPVPYTGAVK.V

K.QGQDNLSSVK.E

K.WAEQYLK.I

K.SLNILTAFQK.K

 

22

ATP5H_HUMAN

ATP synthase subunit d, mitochondrial

18.5/5.21

6

40.00

K.TIDWVAFAEIIPQNQK.A

K.SWNETLTSR.L

R.LAALPENPPAIDWAYYK.A

K.AGLVDDFEK.K

K.YTAQVDAEEK.E

K.YTAQVDAEEKEDVK.S

-1.08

 

gi|189054178

unnamed protein product

66.0/7.62

3

5.00

K.SLNNQFASFIDK.V

R.SLDLDSIIAEVK.A

K.LALDLEIATYR.T

 

23

B2MG_HUMAN

Beta-2 microglobulin

12.7/5.77

2

18.00

R.VNHVTLSQPK.I

K.VEHSDLSFSK.D

1.35

24

NDUBA_HUMAN

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

20.8/8.60

3

20.35

(K)AFDLIVDRPVTLVR(E)

(K)EVEQFTQVAK(A)

(R)YQDLGAYSSAR(K)

-1.05

 

gi|189054178

unnamed protein product

65.9/7.62

7

12.00

R.TNAENEFVTIK.K

R.SLDLDSIIAEVK.A

K.YEELQITAGR.H

K.LNDLEDALQQAK.E

K.LALDLEIATYR.T

R.TLLEGEESR.M

R.GSGGGSSGGSIGGR.G

 

25

ASCC1_HUMAN

Activating signal cointegrator 1 complex subunit 1

45.48/5.22

1

1.75

(R)SFALLPR(L)

1.11

 

PHB_HUMAN

prohibitin

29.8/5.57

11

52.00

K.FGLALAVAGGVVNSALYNVDAGHR.A

K.DLQNVNITLR.I

R.FDAGELITQR.E

R.AATFGLILDDVSLTHLTFGK.E

K.EFTEAVEAK.Q

K.QVAQQEAER.A

K.AAIISAEGDSK.A

K.AAELIANSLATAGDGLIELR.K

R.KLEAAEDIAYQLSR.S

K.LEAAEDIAYQLSR.S

R.NITYLPAGQSVLLQLPQ.-

 

26

PHB_HUMAN

Prohibitin

29.8/5.57

13

59.00

K.VFESIGK.F

K.DLQNVNITLR.I

R.ILFRPVASQLPR.I

R.IFTSIGEDYDER.V

R.VLPSITTEILK.S

R.FDAGELITQR.E

R.AATFGLILDDVSLTHLTFGK.E

K.EFTEAVEAK.Q

K.QVAQQEAER.A

K.AAIISAEGDSK.A

K.AAELIANSLATAGDGLIELR.K

R.KLEAAEDIAYQLSR.S

R.NITYLPAGQSVLLQLPQ.-

1.06

 

NDUS3_HUMAN

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

30.2/6.99

2

9.00

K.SLVDLTAVDVPTR.Q

K.DFPLSGYVELR.Y

 
  1. Note: Spot no. 1, 3, 5, 7, 13 and 14 were matched from our hepatocellular carcinoma cell line database. *The density of spots were calculated as percent volume and shown in this table as folds of increase or decrease (-).