Figure 4

Detection of expression of HDAC4, HDAC1, and HDAC2 in various bladder cancer cells. The cell lysates prepared from the HTB2, HTB3, HTB4, HTB5, HTB9, HT1197, HT1376 bladder cancer cells and GM00637 normal human fibroblast cells (30 μg total protein) were analyzed by western blots to determine the protein levels of HDAC4, HDAC1, HDAC2, and β-actin in each cell lysate. The antibodies against HDAC4 (A-4), HDAC1 (C-19), HDAC2 (H-54) and β-actin (C-2) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) and used in the western blots study.