Figure 5

The cisplatin-induced caspase 3 activity in both untreated and VPA-treated HTB4 and HTB9 bladder cancer cells. The VPA treatment (5 mM) was done 24 hours prior to the cisplatin treatment. The cells were treated with cisplatin at the indicated concentrations for 3 hours and then cultured in the cell culture incubator for 40 hours before the cells were harvested and the caspase 3 activity was measured. The caspase 3 activity was measured as nanomole of AMC/minute/mg of protein. (* statistical difference to that of the untreated cells with p value < 0.01).