Schematic representation of plasmid constructs used in the present study. The structural pattern of two types of expression cassettes of TCR α6- and β13-chain genes. The TCRα- and β-chain genes were introduced into the pIRES vector and linked by an IRES element. For both linker elements, TCRα was integrated into either the 5' position (αIβ) or the 3' position (βIα). A) TCR Vα6-IRES-TCR Vβ13 recombinant plasmid. B) TCR Vβ13-IRES-TCR Vα6 recombinant plasmid. C) The structure of cassettes αIβ and βIα.