Specific cytotoxicity of TCR gene-transduced CD3+T cells directed against Toledo cells as determined by LDH release assay. Three days after transduction, TCR gene-transduced CD3+T cells by two different TCR transgene cassettes were cocultured with Toledo, Raji, or Molt-4 cells at a 10:1 ratio for 10 h. Then, the LDH level in the supernatant was determined. The spontaneous release of LDH from both target and effector cells was subtracted from the measured values and the final results are expressed as the percentage of specific cytotoxicity. *Mann-Whitney test of two independent samples was used to determine differences between the various groups. Statistical significance was defined as P < 0.05.