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Table 1 The sequence of primers for PCR

From: Generation of diffuse large B cell lymphoma-associated antigen-specific Vα6/Vβ13+T cells by TCR gene transfer

Primers

Function

Sequences

Vα6

Sense primer for TCR α6 genes

5'-TCCGCCAACCTTGTCATCTCCGCT-3'

Antisense primer for TCR α6 genes

5'-GTTGCTCCAGGCCGCGGCACTGTT-3'

Vβ13

Sense primer for TCR β13 genes

5'-CACTGCGGTGTACCCAGGATATGA-3'

Antisense primer for TCR β13 genes

5'-CGGGCTGCTCCTTGAGGGGCTGCG-3'

VA6-F

Sense primer for full-length TCR α6 genes

5'-GCCAGGTTCACCTCACAGTACAGAGTCC-3'

VA6-R

Antisense primer for full-length TCR α6 genes

5'-GCAGAGGAAGGAGCGAGGGAGCAC-3'

VB13-F

Sense primer for full-length TCR β13 genes

5'-GCACAGATACAGAAGACCCCTCCGTC-3'

VB13-R

Antisense primer for full-length TCR β13 genes

5'-GGGTGAGGATGAAGAATGACCTGGGATG-3'

VA6-EF

Sense primer for TCR α6 genes in the 5' position of IRES

5'-ACGGAATTC GCCAGGTTCACCTCACAGTACAGAG-3'

VA6-MR

Antisense primer for TCR α6 genes in the 5' position of IRES

5'-TCGACGCGT TCA GAGGAAGGAGCGAGGGAGCAC-3'

VB13-SF

Sense primer for TCR β13 genes in the 3' position of IRES

5'-TTAGTCGAC GCACAGATACAGAAGACCCCTCCGTC-3

VB13-NR

Antisense primer for TCR β13 genes in the 3' position of IRES

5-'TAATGCGGCCGC TCA TGAGGATGAAGAATGACCTGGGATG-3'

VB13-EF

Sense primer for TCR β13 genes in the 5' position of IRES

5'-CGGAATTC GCACAGATACAGAAGACCCCTCCGTC-3'

VB13-MR

Antisense primer for TCR β13 genes in the 5' position of IRES

5'-TCCACGCGT TCA GTGAGGATGAAGAATGACCTGGGATG-3'

VA6-SF

Sense primer for TCR α6 genes in the 3' position of IRES

5'-TTGGTCGAC GCCAGGTTCACCTCACAGTACAGAG-3'

VA6-NR

Antisense primer for TCR α6 genes in the 3' position of IRES

5'-TAATGCGGCCGC TCA GAGGAAGGAGCGAGGGAGCAC-3'

  1. Note: Boldface with underline indicates restriction site; Italic with shadow indicates stop codon.