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Table 1 The sequence of primers for PCR

From: Generation of diffuse large B cell lymphoma-associated antigen-specific Vα6/Vβ13+T cells by TCR gene transfer

Primers Function Sequences
Vα6 Sense primer for TCR α6 genes 5'-TCCGCCAACCTTGTCATCTCCGCT-3'
Antisense primer for TCR α6 genes 5'-GTTGCTCCAGGCCGCGGCACTGTT-3'
Vβ13 Sense primer for TCR β13 genes 5'-CACTGCGGTGTACCCAGGATATGA-3'
Antisense primer for TCR β13 genes 5'-CGGGCTGCTCCTTGAGGGGCTGCG-3'
VA6-F Sense primer for full-length TCR α6 genes 5'-GCCAGGTTCACCTCACAGTACAGAGTCC-3'
VA6-R Antisense primer for full-length TCR α6 genes 5'-GCAGAGGAAGGAGCGAGGGAGCAC-3'
VB13-F Sense primer for full-length TCR β13 genes 5'-GCACAGATACAGAAGACCCCTCCGTC-3'
VB13-R Antisense primer for full-length TCR β13 genes 5'-GGGTGAGGATGAAGAATGACCTGGGATG-3'
VA6-EF Sense primer for TCR α6 genes in the 5' position of IRES 5'-ACGGAATTC GCCAGGTTCACCTCACAGTACAGAG-3'
VA6-MR Antisense primer for TCR α6 genes in the 5' position of IRES 5'-TCGACGCGT TCA GAGGAAGGAGCGAGGGAGCAC-3'
VB13-SF Sense primer for TCR β13 genes in the 3' position of IRES 5'-TTAGTCGAC GCACAGATACAGAAGACCCCTCCGTC-3
VB13-NR Antisense primer for TCR β13 genes in the 3' position of IRES 5-'TAATGCGGCCGC TCA TGAGGATGAAGAATGACCTGGGATG-3'
VB13-EF Sense primer for TCR β13 genes in the 5' position of IRES 5'-CGGAATTC GCACAGATACAGAAGACCCCTCCGTC-3'
VB13-MR Antisense primer for TCR β13 genes in the 5' position of IRES 5'-TCCACGCGT TCA GTGAGGATGAAGAATGACCTGGGATG-3'
VA6-SF Sense primer for TCR α6 genes in the 3' position of IRES 5'-TTGGTCGAC GCCAGGTTCACCTCACAGTACAGAG-3'
VA6-NR Antisense primer for TCR α6 genes in the 3' position of IRES 5'-TAATGCGGCCGC TCA GAGGAAGGAGCGAGGGAGCAC-3'
  1. Note: Boldface with underline indicates restriction site; Italic with shadow indicates stop codon.