Figure 1

ATRA inhibits proliferation and induces differentiation in HL-60 cells. (A) Morphology of HL-60 cells treated with 0.1 μmol/L ATRA or 0.1% alcohol at 48 hours. (B) Cell inhibition rates in ATRA and 0.1% alcohol treated HL-60 cells at 24-72 hours, determined using the MTT assay. Each group was assayed in triplicate. Cell inhibition rate was calculated as 100% × (control group A values -experimental group A values)/control group A values. (C-E) HL-60 cell differentiation was assessed using CD11b and the NBT reduction test. (C-D) CD11b flow cytometry analysis of cells treated with ATRA or 0.1% alcohol. The expression rate was determined as the number of CD11b positive cells in 1 × 10⁴ cells. (E) NBT analysis of HL-60 cells treated with ATRA or 0.1% alcohol. Two hundred cells were observed and positive cells with blue formazan crystals were counted by microscopy, **p < 0.01.