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Figure 4 | Journal of Hematology & Oncology

Figure 4

From: Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663

Figure 4

Lentivirus expressing miR-663 induces HL-60 cell differentiation. A lentivirus miR663 expressing (LV-miR-663) construct was generated. (A) Multiplicities of infection (MOI) of 1, 10 and 100 were used to determine optimal transduction efficiency in HL-60 cells using a control GFP-expressing lentivirus; GFP was detected in approximately 80% cells in the MOI 100 group. (B) MTT assays indicated the inhibition rates of HL-60 transfected with LV-miR-663 were 34.2 ± 13.1%, 45.2 ± 24.5% and 53.2 ± 21.3% at 1, 2 and 3 days, respectively, compared with mock transfected cells, p < 0.01. These results indicate miR-663 expression inhibits HL-60 proliferation in a time-dependent manner. (C) miR-663 induces HL-60 differentiation to mature granulocytes. In cells transfected with LV-miR-663 expression of the differentiation marker CD11b was increased significantly and after 1, 2 or 3 days the expression rates of CD11b were 21.2 ± 9.3%, 27.4 ± 12.5% and 33.2% ± 12.4% respectively in LV-miR-663 transfected cells, compared with the mock transfected cells (0.56 ± 0.21%, p < 0.01).

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