Figure 2
From: Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4
Flow cytometry assays by using individual antibodies revealed that most Sall4A or Sall4B treated LSK cells retained immature surface phenotypes after 2 weeks culture, while GFP control LSK cells exhibited increased lineage differentiation. The lineage cocktail consists of antibodies against CD5, CD45R, CD11b, Ter119, and GR-1. Experiments were repeated at least four times and a representative case is shown.