Figure 3
From: Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4
Enhanced HSC/HPC activity by forced expression of Sall4 isoforms. GFP control or Sall4 -transduced LSK cells (Ly5.2+, 2 × 106 cells, day-10) were transplanted into sublethally irradiated mice in combination with 1 × 105 normal BM cells with Ly5.1 phenotype. Four mice received GFP or Sall4A treated LSK cells and three mice received Sall4B treated LSK cells. (a) The population of donor cells (Ly5.2+) in the peripheral blood of the recipient mice was examined by flow cytometry assays with the indicated antibodies at 2 weeks after BM transfusion. (b) The Ly5.2+ cell percentages within the peripheral white blood cells of recipients were measured at 2, 20, and 50 weeks after transplantation of indicated lentivirus-infected BM cells. Error bars represent standard error of the mean.