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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Relevance of JAK2V617F positivity to hematological diseases - survey of samples from a clinical genetics laboratory

Figure 2

Comparison of JAK2V617F detections by using direct and nested allele-specific PCR with non-purified and purified DNA samples. Non-purified and phenol/chloroform extraction-purified DNAs from JAK2V617F-negative (lane A) and JAK2V617F-positive (lane B) samples were subjected to direct or nested allele-specific PCR analyses as indicated. The final PCR products were analyzed on 3% agarose and visualized with ethidium bromide staining. Note that the direct PCR analyses of purified DNAs and the nested PCR analyses of non-purified DNAs gave rise to consistent results while the direct PCR of non-purified DNAs did not yield any PCR product.

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