Figure 2
From: Development of a highly sensitive method for detection of JAK2V617F
Validation of the RFN-AS-PCR method by using mixtures of DNA samples from normal and MPN blood samples. Blood cell DNAs from a heterozygous JAK2V617F-positive ET patient and a normal donor were mixed in the indicated proportions. Initial PCR was performed with primers P1 and P1r, and PCR products were directly subjected to nested AS-PCR (panel A) or digested with BsaXI and then subjected to nested AS-PCR (panel B). Note that the BsaXI digestion increased the detection sensitivity from 0.1% to 0.001% ET blood.