Figure 1
From: Impact of bile acids on the growth of human cholangiocarcinoma via FXR
Bile acids regulated the expression of FXR. A, RT-PCR analysis showed that free bile acids (CA, DCA, CDCA) enhanced the mRNA expression of FXR whereas conjugated bile acids (GCA, GDCA, GCDCA) decreased FXR mRNA level. GAPDH served as the loading control. B, Realtime-PCR analysis showed that differences of FXR mRNA expression according to density level after CDCA and GDCA treatment. β-actin served as an internal control. C, The protein expression of FXR was tested by Western blot. Free bile acids increased the expression of FXR, while conjugated bile acids decreased the expression of the FXR protein. TBP (TATA-binding protein, a kind of nucleoprotein) served as the loading control.