FXR antagonist/agonist changed the effects of bile acids on FXR expression. A, combination of GS (FXR antagonist) or GW 4064 (FXR agonist) with free bile acids. B, combination of GS or GW 4064 with conjugated bile acids. C, bile acids and FXR antagonist/agonist changed the expression of FXR. TBP: TATA-binding protein (loading control). * p < 0.05, compared with the control group. # p < 0.05, compared with bile acid treatment groups.