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Figure 3 | Journal of Hematology & Oncology

Figure 3

From: De novo acute megakaryoblastic leukemia with p210 BCR/ABL and t(1;16) translocation but not t(9;22) Ph chromosome

Figure 3

Detection of fusion genes. a. Multiplex reverse transcription-PCR analysis showed that the cells of this patient were positive for a translocation in multiplex reaction 6 (The arrow). Internal positive control: E2A (690 bp). The primer sets used in multiplex reverse transcription-PCR were chosen as described previously [11] to detect the following fusion genes: 1. CBFβ/MYH11/MLL/AFX1/AF6/ELL/E2A; 2. MLL/AF1P/AF17/AF10/E2A; 3. PBX1/SIL/TAL1/HLF/TEL/AML1A/E2A; 4. AML1A/AMLMDS EVI/HOX11/ETO/TLS/ERG/E2A; 5. MLL/AF4/AF9/AF1Q/ENL/E2A; 6. BCR/ABL/TEL/E2A; 7. DEK/CAN/SET/AMLMDSEVI /E2A; 8. PLZF/PML3/RARα/NPM/NPMALK /MLF1/E2A; b. The split-out PCR analysis revealed a BCR/ABL b2a2 fusion gene expression using primer sets for 1. BCR/ABL e1a2 (320 bp); 2. BCR/ABL b2a2 (472 bp) and BCR/ABL b3a2 (397 bp); 3. TEL/ABL (366 bp); c. Fluorescence in situ hybridization (FISH) using a Vysis LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe (Vysis, Downers Grove, IL, USA) on an interphase cell. Orange signal is ABL gene on chromosome 9 and green signal is BCR gene on chromosome 22. The ABL orange signals occurred on both chromosomes 9 and on der(9)ins(22;9) and one BCR green signal on chromosomes 22 and one yellow fusion signal on der(22)ins(22;9). The arrow indicates the fusion signal.

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