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Table 1 Spleen cells were isolated and cultured for 72 hours under the indicated conditions with the following concentrations: IL-2 (100 u/ml), TGFβ (10 ng/ml) and αCD3/αCD28 (1 μg/ml immobilized, 10 μg/ml soluble, respectively).

From: T cell receptor transgenic lymphocytes infiltrating murine tumors are not induced to express foxp3

Culture Conditions C57BL/6 Foxp3EGFP
CD8
C57BL/6 Foxp3EGFP
CD4
Pmel Foxp3EGFP
CD8
Pmel
Foxp3EGFP
CD4
OTII Foxp3EGFP
CD4
Media Only 0.9 +/- 0.6 2.1 +/- 1.3 2.2 +/- 0.1 9.6 +/- 5.9 0.8 +/- 0.1
IL-2 1.9 +/- 0.5 8.2 +/- 1.8 2.9 +/- 0.1 11.7 +/- 1.3 0.6 +/- 0.3
TGF- β 1.8 +/- 0.1 4.2 +/- 1.1 4.8 +/- 0.8 13.2 +/- 1.6 0 +/- 0.6
IL-2/TGF-β 2.0 +/- 1.3 20.4 +/- 1.4 1.4 +/- 0.8 20.7 +/- 3.4 0.8 +/- 0.5
αCD3/αCD28 0.9 +/- 1.8 3.1 +/- 0.2 2.3 +/- 0.3 17.2 +/- 1.7 3.0 +/- 0.1
αCD3/αCD28/IL-2 1.3 +/- 1.6 4.6 +/- 0.7 21.3 +/- 0.8 13.8 +/- 0.5 3.0 +/- 0.2
αCD3/αCD28/TGF-β 18.8 +/- 3.0 18.8 +/- 1.0 27.3 +/- 1.8 30.7 +/- 2.8 7.7 +/- 2.4
αCD3/αCD28/IL-2/TGFβ 25.1 +/- 0.4 34.4 +/- 1.1 27.9 +/- 0.2 46.2 +/- 1.1 8.4 +/- 0.4
  1. Cells were then sorted for CD8, CD4, and EGFP expression. Results are a mean of triplicates +/- S.D. These experiments were repeated at least 3 times with comparable results.