Figure 3
Dot plot analysis of 5-MF or 2'-MF or DMF or TMF or PMF-treated MOLT-4 cells in the presence or absence of TRAIL. The concentrations of 5-MF and TRAIL used were at IC20 levels. (a) The cells were untreated (A), treated with TRAIL for 24 h (B), 5-MF for 3 h (C), 5 MF for 3 h and TRAIL for 24 h (D), 5-MF for 6 h (E), 5-MF for 6 h and TRAIL for 24 h (F), 5-MF for 12 h (G), 5-MF for 12 h and TRAIL for 24 h (H), 5-MF for 18 h (I), 5-MF for 18 h and TRAIL for 24 h (J), 5-MF for 24 h (K) and 5-MF for 24 h and TRAIL for 24 h (L). (b) The MOLT-4 cells were untreated (A), treated with TRAIL for 24 h (B), 2'-MF for 3 h (C), 2'-MF for 3 h and TRAIL for 24 h (D), 2'-MF for 6 h (E), 2'-MF for 6 h and TRAIL for 24 h (F), 2'-MF for 12 h (G), 2'-MF for 12 h and TRIAL for 24 h (H), 2'-MF for 18 h (I), 2'-MF for 18 h and TRIAL for 24 h (J), 2'-MF for 24 h (K) 2'-MF for 24 h and TRAIL for 24 h (L). (c) The MOLT-4 cells were untreated (A), treated with TRAIL for 24 h (B), DMF for 24 h (C), DMF for 24 h plus TRAIL for 24 h (D), TMF for 24 h (E), TMF for 24 h plus TRAIL for 24 h (F), PMF for 24 h (G) and PMF for 24 h plus TRAIL for 24 h (H). The cells were stained with annexin V-FITC and PI, and processed by using flow cytometer. The percentages of early, late apoptotic cells and necrotic cells are shown in the right lower quadrant, right upper quadrant and left upper quadrant, respectively.