Figure 1From: Isolation, characterization, and in vitro propagation of infantile hemangioma stem cells and an in vivo mouse modelExpression of SALL4 and CD133 in IH tissues. (A) Immunohistochemistry staining of proliferative (1,3) and involuting (2,4) IH tissue sections with anti-SALL4 and CD133 antibodies. Magnification: 100x, inset 200x. (B) 1, Phase contrast image of IHs tumor spheres forming on Petri dishes with surrounding mature endothelial cells and/or fibroblasts. This was observed after 3 days of culture in serum-free media. Magnification: 100 ×. 2, the tumor spheres continue to grow under serum-free conditions while mature cells such as endothelial cells and fibroblasts do not. Magnification: 100x. 3, phase contrast image of high density tumor spheres in culture. Magnification: 100 ×. 4, comparison of IH tumor sphere (left) and induced pluripotent stem cell clone (right). Magnification: 100 ×.Back to article page