Figure 8
From: BCR-ABL1-independent PI3Kinase activation causing imatinib-resistance
PI3Kα E545G mutation in cell line KCL-22. Genomic sequencing and cDNA sequencing (shown here) showed that cell line KCL-22 heterozygously carried PI3Kα E545G. No mutations were found in the helical (exon 10) and kinase domains (exon 21) of the remaining TKI-resistant cell lines.