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Table 2 Effect of rapamycin on proliferation of BCR-ABL1-positive cell lines

From: BCR-ABL1-independent PI3Kinase activation causing imatinib-resistance

  [3H]-thymidine (SI) % cells in G1
  control rapamycin control rapamycin
TKI-sensitive    
BV-173 1 0.3 +/- 0.1 63 +/- 4 69 +/- 4
EM-2 1 0.5 +/- 0.1 61 +/- 2 68 +/- 1
JURL-MK2 1 0.6 +/- 0.1 73 +/- 1 81 +/- 2
TKI-resistant    
KCL-22 1 0.4 +/- 0 60 +/- 5 74 +/- 2
NALM-1 1 0.6 +/- 0.1 76 +/- 2 80 +/- 1
SUP-B15 1 0.5 +/- 0.1 66 +/- 1 83 +/- 2
  1. Proliferation was assessed applying the [3H]-thymidine incorporation assay. Stimulation index (SI) was determined setting uptake (cpm) of untreated cells to 1. Shown are results of three experiments, each done in triplicate. Cell cycle analysis was performed by flow cytometry with ethanol-fixed, PI stained cells. Experiments were performed in triplicates. Both assays were performed after 24 h with/without rapamycin (10 nM).