Figure 3

Detection of T315I in CML patient samples by DHPLC, sequencing and AS-PCR; Figure 3A shows DHPLC patterns followed by sequencing analysis if a suspicious peak was observed; T315I cell lines and wild types are also shown; Figure 3B demonstrates representative AS-PCR results of four CML cases with abnormal DHPLC and sequencing results (patients no.360, no.461, no.504, and no. 509) .