Figure 1

TF promoter activity in MDA-MB-231-TFluc cells treated by LY294002, wortmannin and PD98059. Panel a: MDA-MB-231-TFluc cells were treated by PD98059 at 10 μM for different time periods, then washed. Panels b-c: the cells were treated by LY294002 (b), wortmannin (c) and PD98059 (d) at indicated concentrations for 24 hrs. The activity of luciferase in the cells was measured. The data represent the means values of three independent experiments.