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Figure 3 | Journal of Hematology & Oncology

Figure 3

From: Opposite regulation by PI3K/Akt and MAPK/ERK pathways of tissue factor expression, cell-associated procoagulant activity and invasiveness in MDA-MB-231 cells

Figure 3

TF promoter activity, TF mRNA and Akt phosphorilation after combined treatments. Panel a: MDA-MB-231-TFluc cells were treated by 10 μM of LY294002 and 0.1 μM of wortmannin, in the absence or the presence of 10 μM PD98059 for 24 hrs. The activity of luciferase was measured. The data was represented as means±SEM of three independent experiments. * : p<0.05. Panel b: qPCR results of TF mRNA (Hs00175225_m1) in the cells after the treatment for 24 hrs by 10 μM of LY294002, 10 μM of A6730, 0.1 μM of wortmannin, in the absence or the presence of 10 μM PD98059. The experiments were repeated for 3 times. ** : p<0.05. Panel c: MDA-MB-231 cells were treated with 0.1 μM of wortmannin, 10 μM of LY294002 in the presence or the absence of PD98059 at 10 μM or 50 μM for 24 hrs. Total protein was extracted for the determination of pAkt level by western blot using anti-Akt or anti-pAkt antibodies. The data were from three blots. * : p<0.05.

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