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Figure 4 | Journal of Hematology & Oncology

Figure 4

From: Cytoreduction surgery reduces systemic myeloid suppressor cell populations and restores intratumoral immunotherapy effectiveness

Figure 4

Intratumoral immunotherapy prior to surgery is effective due to enhanced CD8 T-cell function. (A) in vivo tumor neutralization assay. Mice bearing established flank tumors were randomized to one of four groups: (i) Ad.LacZ/GCV, (ii) AdV-tk/GCV, (iii) Ad.LacZ/GCV with cytoreduction surgery, and (iv) AdV-tk/GCV with cytoreduction surgery. CD8 T-cells were mixed with fresh tumor cells and injected into tumor naïve mice. After 9 days of tumor growth, we found that CD8 T-cells harvested from mice receiving AdV-tk/GCV and cytoreductive surgery inhibited tumor growth more potently than CD8 T-cells from other treatment groups. (B) Immunohistochemistry images of representative tumor from mice receiving AdV-tk/GCV or Ad.LacZ/GCV. Specimens were stained using anti-CD8 antibodies.

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