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Figure 1 | Journal of Hematology & Oncology

Figure 1

From: Effect of phenylhexyl isothiocyanate on aberrant histone H3 methylation in primary human acute leukemia

Figure 1

Histone H3 methylation in primary human leukemia cells. The methylation status of histone H3 lysine 4 (H3K4) and H3 lysine 9 (H3K9) was examined by Western blotting. Panel A, top group, shows the expression level of methylated H3K4 and H3K9 in primary marrow mononuclear cells isolated from seven acute leukemia cases (P1-P7), one individual without leukemia (N1), and one healthy volunteer (V1). The middle and lower groups of panel A depict the level of methylated H3K4 and H3K9 in the cells from additional twelve acute leukemia cases (P8-P19), non-leukemia individual with IDA (N2), one with infection (N3), two with ITP (N4, N5), and two healthy volunteers (V2, V3). β-actin was used as a loading control. The expression level of the methylated H3K4 (Panel B) and H3K9 (panel C) in primary leukemia cells from all leukemia patients (P1-P19) was compared with those of non-leukemia cells (N1-N5 and V1-V3). The vertical bars in panels B and C represent the mean ± SD of the densities of methylated H3K4 and H3K9, quantified as densitometric unit value in absorbance from the Western blottings in panel A. (▓) indicates the level of methylated H3K4 (panel B) and H3K9 (panel C) from the 19 leukemia patients (P1-P19), and (□) indicates the level of methylated H3K4 and H3K9 from the 8 non-leukemia subjects (N1-N5, V1-V3). Panel B shows that the histone H3K4 in acute leukemia cells is significantly lower than that in the non-leukemia cells (P < 0.05). Panel C shows that the histone H3K9 in acute leukemia cells is significantly higher than that in the non-leukemia cells (P < 0.05).

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