Figure 1

Detection of IDH1 and IDH2 mutations by DHPLC and direct sequencing analysis. DHPLC patterns shows a suspicious peak in cases with IDH1 or IDH2 mutations as compared to cases with the wild type (WT); Six missense IDH1 mutations; R132H, R132C, R132S, R132G, R132L, and I99M and 1 silent mutation (G105G), and 2 missense IDH2 mutations; R140Q and R172K, are demonstrated. (Abbreviation: C, cysteine; G, glycine; H, histidine; I, isoleucine; K, lysine; L, leucine; M, methionine; Q, glutamine; S, serine).